Broadband Coherent Anti-Stokes Raman scattering (BCARS)
For the multiparametric assessment of the structural and metabolic modifications we established in our laboratory a BCARS microscope.
The method detects with high specificity the chemical fingerprints of cells, tissues or biofluids.
BCARS is based on the detection of the fingerprint region of molecules (500-1500 cm-1) and could probe multiple Raman transitions simultaneously, to allow a “chemical” imaging of biological tissues with improved molecular contrast.
Spinal cord injury in a murine model imaged by Broadband Coherent Raman Spectroscopy (BCARS) microscope (violet- lipids, myelin, cyan- cell nuclei, green- protein network)
BCARS spectra recorded on lesioned mice spinal cord tissue
BCARS images of dental pulp stem cells and red blood cells in a fibrin hydrogel: a dental pulp regeneration model