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(218) Production(s) de GERGELY C.
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Déposition des cellules pulpaires humaines et des multicouches de polyelectrolites (MPEs) par spray pour la fonctionnalisation des biomatériaux.
Auteur(s): Panayotov Ivan, Martin Fernandez M., Yachouh J., Gergely C., Vladimirov B., Cuisinier Frédéric J. G.
Conference: IFRO (, FR, 2012-01)
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Fonctionnalisation du titane par des peptides spécifiques bifonctionnels pour l'adhésion épithéliale
Auteur(s): Panayotov Ivan, Vegh Attila Gergely, Martin Fernandez M., Collart-Dutilleul Pierre-Yves, Gergely C., Cuisinier Frédéric J. G.
Conference: 3ème Journée Recherche en Odontologie (Marseille, FR, 2012-06)
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Fonctionnalisation de la surface du Titane et du Ti6Al4V par des peptides spécifiques bi-fonctionnels pour l'adhésion épithéliale.
Auteur(s): Panayotov Ivan, Martin Fernandez M., Yachouh J., Gergely C., Vladimirov B., Cuisinier Frederic J. G.
Conference: GIRSO (Penafiel, PT, 2012)
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Light-harvesting bio-nanomaterial using porous silicon and photosynthetic reaction center
Auteur(s): Hajdu Kata, Gergely C., Martin Fernandez M., Zimanyi Laszlo, Agarwal Vivechana, Palestino Gabriela, Hernadi Klara, Németh Zoltán, Nagy Laszlo
(Article) Publié:
Nanoscale Research Letters, vol. 7 p.400 (2012)
Texte intégral en Openaccess :
Ref HAL: hal-00732386_v1
PMID 22804837
DOI: 10.1186/1556-276X-7-400
WoS: 000309159800001
Exporter : BibTex | endNote
12 Citations
Résumé: Porous silicon microcavity (PSiMc) structures were used to immobilize photosynthetic reaction center (RC) purified from the purple bacterium Rhodobacter sphaeroides R-26. Two different binding methods were compared by specular reflectance measurements. Structural characterization of PSiMc was performed by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The activity of the immobilized RC was checked by measuring the visible absorption spectra of the externally added electron donor, mammalian cytochrome c. PSi/RC complex was found to oxidize the cytochrome c after every saturating Xe flash, indicating the accessibility of specific surface binding sites on the immobilized RC, for the external electron donor. This new type of bio-nanomaterial is considered as an excellent model for new generation applications of silicon based electronics and biological redox systems.
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Multiphoton imaging of the dentine-enamel junction
Auteur(s): Cloitre T., Panayotov Ivan, Tassery Hervé, Gergely C., Levallois Bernard, Cuisinier Frederic J. G.
(Article) Publié:
Journal Of Biophotonics, vol. p.DOI 10.1002/jbio.201200065 (2012)
Texte intégral en Openaccess :
Ref HAL: hal-00732326_v1
PMID 22815209
DOI: 10.1002/jbio.201200065
WoS: 000316959300009
Exporter : BibTex | endNote
18 Citations
Résumé: Multiphoton microscopy has been used to reveal structural details of dentine and enamel at the dentin-enamel junction (DEJ) based on their 2-photon excited fluores- cence (2PEF) emission and second harmonic generation (SHG). In dentine tubule 2PEF intensity varies due to protein content variation. Intertubular dentin produces both SHG and 2PEF signals. Tubules are surrounded by a thin circular zone with a lower SHG signal than the bulk dentine and the presence of collagen fibers perpen- dicular to the tubule longitudinal axis is indicated by strong SHG responses. The DEJ appears as a low inten- sity line on the 2PEF images and this was never pre- viously reported. The SHG signal is completely absent for enamel and aprismatic enamel shows a homogeneous low 2PEF signal contrary to prismatic enamel. The SHG intensity of mantle dentine is increasing from the dentine-enamel junction in the first 12 mm indicating a progressive presence of fibrillar collagen and corresponding to the more external part of mantle dentine where matrix metallo-proteases accumulate. The high information content of multiphoton images confirms the huge potential of this method to investigate tooth structures in physiological and pathological conditions. Dentine-Enamel Junction. Superposition of SHG and 2PEF images showing the relationship of enamel and dentine.
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Effect of surface functionalization of porous silicon microcavities on biosensing performance.
Auteur(s): Martin Fernandez M., Massif Laurent, Estephan Elias, Saab Marie-Belle, Cloitre T., Larroque Christian, Agarwal Vivechana, Cuisinier Frédéric J. G., Le Lay Guy, Gergely C.
(Article) Publié:
Journal Of Nanophotonics, vol. 6 p.061506 (2012)
Ref HAL: hal-00714668_v1
DOI: 10.1117/1.JNP.6.061506
WoS: 000304037800003
Exporter : BibTex | endNote
3 Citations
Résumé: Surface functionalization methods were investigated for their effects on the sensing performances of porous silicon (p-Si) microcavities when used for detection of biomolecules. These microcavities were fabricated to reveal reflectivity pass-band spectra in the visible and near-infrared spectral regime. In one approach, the devices were thermally oxidized and functionalized to ensure covalent binding of molecules. In the second approach, the as-etched p-Si surface was modified with adhesion peptides, isolated via phage display, that present high binding capacity for silicon. Functionalization and molecular binding events were monitored via reflectometric interference spectra as shifts in the resonance peaks of the cavity structure due to changes in the refractive index when a biomolecule is attached to the large internal surface of p-Si. Improved sensitivity was obtained owing to the peptide interface linkers between the p-Si and biological molecules compared to the silanized devices. Investigating the formation of pep- tide-Si interface layer via X-ray photoelectron spectroscopy, scanning tunneling microscopy, and scanning electron microscopy, we found that peptides form nanometer-thin layers on the Si surface and that their binding energy depends on the sequence of the peptide.
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Towards the understanding of peptide/inorganic semiconductor complex interaction using molecular dynamics simulations
Auteur(s): Ramakrishnan S., Martin Fernandez M., Cloitre T., Gergely C.
Conference: Porous Semiconductors - Science and Technology (Malaga, ES, 2012-03-25)
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