Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity Auteur(s): Martin Fernandez M., Palestino Gabriela, Agarwal Vivechana, Cloitre T., Zimanyi Laszlo, Gergely C. (Article) Publié: Applied Physics Letters, vol. 94 p.223313 (2009) Ref HAL: hal-00391043_v1 DOI: 10.1063/1.3148698 WoS: 000266674300080 Exporter : BibTex | endNote 6 Citations Résumé: Infiltration of biomacromolecules into porous silicon photonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of optical and structural studies. Nonlinear optical microscopy is applied to study porous silicon microcavities with and without infiltrated glucose oxidase. The infiltrated protein acts as an internal two-photon-excited fluorescence emitter and second harmonic generator, enabling the in-depth visualization of the porous structure. Enhanced second harmonic generation and fluorescence emission by the porous silicon structure is experimentally associated with the defect layer. |