- Laser Doppler holographic microscopy in transmission: application to fish embryo imaging doi link

Auteur(s): Verrier N., Alexandre D., Gross M.

(Article) Publié: Optics Express, vol. 22 p.9368 (2014)
Texte intégral en Openaccess : arxiv

Ref HAL: hal-00970195_v1
PMID 24787825
Ref Arxiv: 1404.7781
DOI: 10.1364/OE.22.009368
WoS: 000335902200054
Ref. & Cit.: NASA ADS
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We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and the combination of frames used to calculate the hologram, multimodal imaging has been performed. Doppler images of the blood vessels for different Doppler shifts, images where the flow direction is coded in RGB colors or movies showing blood cells individual motion have been obtained as well. The ability to select the Fourier space zone that is used to calculate the signal, makes the method quantitative.