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Inferring interphase chromosomal structure from multiplexed fluorescence in situ hybridization data: a unified picture from human and mouse cells ![]() Auteur(s): Remini L., Segers Midas, Parmeggiani A., Carlon Enrico (Article) Publié: The Journal Of Chemical Physics, vol. Volume 162 p. (2025) Ref HAL: hal-04741167_v2 Exporter : BibTex | endNote Résumé: We analyze multiplexed fluorescence in situ hybridization (m-FISH) data for human and mouse cell lines. The m-FISH technique uses fluorescently-labeled single stranded probes which hybridize to specific chromosomal regions, thereby allowing the measurement of the spatial positions of up to ∼ 100 tagged sites for several thousands interphase chromosomes. Our analysis focuses on a wide range of different cell lines and two distinct organisms and provides a unified picture of chromatin structure for scales ranging from 5 kb (kilo bases) up to 2 Mb (mega bases), thus covering a genomic region of almost three orders of magnitude. Confirming recent analysis (Remini et al., Phys. Rev. E 109, 024408 ( 2024)) we show that there are two characteristic arrangements of chromatin referred to as phase α (crumpled globule) and β (looped domain) and discuss the physical properties of these phases. We show that a simple heterogeneous random walk model captures the main behavior observed in experiments and bring considerable insights on chromosomal structure. Commentaires: This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in (citation of published article) and may be found at https://doi.org/10.1063/5.0236067 |