Light-harvesting bio-nanomaterial using porous silicon and photosynthetic reaction center Auteur(s): Hajdu Kata, Gergely C., Martin Fernandez M., Zimanyi Laszlo, Agarwal Vivechana, Palestino Gabriela, Hernadi Klara, Németh Zoltán, Nagy Laszlo (Article) Publié: Nanoscale Research Letters, vol. 7 p.400 (2012) Texte intégral en Openaccess : Ref HAL: hal-00732386_v1 PMID 22804837 DOI: 10.1186/1556-276X-7-400 WoS: 000309159800001 Exporter : BibTex | endNote 12 Citations Résumé: Porous silicon microcavity (PSiMc) structures were used to immobilize photosynthetic reaction center (RC) purified from the purple bacterium Rhodobacter sphaeroides R-26. Two different binding methods were compared by specular reflectance measurements. Structural characterization of PSiMc was performed by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The activity of the immobilized RC was checked by measuring the visible absorption spectra of the externally added electron donor, mammalian cytochrome c. PSi/RC complex was found to oxidize the cytochrome c after every saturating Xe flash, indicating the accessibility of specific surface binding sites on the immobilized RC, for the external electron donor. This new type of bio-nanomaterial is considered as an excellent model for new generation applications of silicon based electronics and biological redox systems. |