Rapid sensing of circulating ghrelin by hypothalamic appetite-modifying neurons. Auteur(s): Schaeffer Marie, Langlet Fanny, Lafont Chrystel, Molino F., Hodson David J, Roux Thomas, Lamarque Laurent, Verdié Pascal, Bourrier Emmanuel, Dehouck Bénédicte, Banères Jean-Louis, Martinez Jean, Méry Pierre-François, Marie Jacky, Trinquet Eric, Fehrentz Jean-Alain, Prévot Vincent, Mollard Patrice (Article) Publié: Proceedings Of The National Academy Of Sciences Of The United States Of America, vol. 110 p.1512-7 (2013) Texte intégral en Openaccess : Ref HAL: hal-00796900_v1 PMID 23297228 DOI: 10.1073/pnas.1212137110 WoS: 000314453900074 Exporter : BibTex | endNote 204 Citations Résumé: To maintain homeostasis, hypothalamic neurons in the arcuate nucleus must dynamically sense and integrate a multitude of peripheral signals. Blood-borne molecules must therefore be able to circumvent the tightly sealed vasculature of the blood-brain barrier to rapidly access their target neurons. However, how information encoded by circulating appetite-modifying hormones is conveyed to central hypothalamic neurons remains largely unexplored. Using in vivo multiphoton microscopy together with fluorescently labeled ligands, we demonstrate that circulating ghrelin, a versatile regulator of energy expenditure and feeding behavior, rapidly binds neurons in the vicinity of fenestrated capillaries, and that the number of labeled cell bodies varies with feeding status. Thus, by virtue of its vascular connections, the hypothalamus is able to directly sense peripheral signals, modifying energy status accordingly. |