Ref HAL: hal-00822202_v1
PMID 23698321
DOI: 10.1117/1.JBO.18.5.056010
WoS: 000322339900025
Exporter : BibTex | endNote
22 Citations
Résumé:

Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cyto- plasm, indicating cell apoptosis via a caspase independent pathway.