Laser Doppler holographic microscopy in transmission: application to fish embryo imaging Auteur(s): Verrier N., Alexandre D., Gross M. (Article) Publié: Optics Express, vol. 22 p.9368 (2014) Texte intégral en Openaccess : Ref HAL: hal-00970195_v1 PMID 24787825 Ref Arxiv: 1404.7781 DOI: 10.1364/OE.22.009368 WoS: 000335902200054 Ref. & Cit.: NASA ADS Exporter : BibTex | endNote 12 Citations Résumé: We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and the combination of frames used to calculate the hologram, multimodal imaging has been performed. Doppler images of the blood vessels for different Doppler shifts, images where the flow direction is coded in RGB colors or movies showing blood cells individual motion have been obtained as well. The ability to select the Fourier space zone that is used to calculate the signal, makes the method quantitative. |