Polydispersity analysis of Taylor dispersion data: the cumulant method Auteur(s): Cipelletti L., Biron Jean-Philippe, Martin Fernandez M., Cottet Hervé (Article) Publié: Analytical Chemistry, vol. 86 p.6471-6478 (2014) Texte intégral en Openaccess : Ref HAL: hal-01058494_v1 Ref Arxiv: 1408.6085 DOI: 10.1021/ac501115y WoS: 000338488800043 Ref. & Cit.: NASA ADS Exporter : BibTex | endNote 23 Citations Résumé: Taylor dispersion analysis is an increasingly popular characterization method that measures the diffusion coefficient, and hence the hydrodynamic radius, of (bio)polymers, nanoparticles or even small molecules. In this work, we describe an extension to current data analysis schemes that allows size polydispersity to be quantified for an arbitrary sample, thereby significantly enhancing the potentiality of Taylor dispersion analysis. The method is based on a cumulant development similar to that used for the analysis of dynamic light scattering data. Specific challenges posed by the cumulant analysis of Taylor dispersion data are discussed, and practical ways to address them are proposed. We successfully test this new method by analyzing both simulated and experimental data for solutions of moderately polydisperse polymers and polymer mixtures. Commentaires: 41 pages (including Supporting Information), 5 figures (+ 7 figs. in the Supporting Information) |