Ref HAL: hal-01407655_v1
DOI: 10.1021/acs.biomac.5b01260
WoS: WOS:000366616700024
Exporter : BibTex | endNote
11 Citations
Résumé:

This work aims at demonstrating the interest ofmodern Taylor dispersion analysis (TDA), performed in narrow internal diameter capillary, for monitoring biopolymer degradations. Hydrolytic and enzymatic degradations ofdendrigraft poly-L-lysine taken as model compounds havebeen performed and monitored by TDA at differentdegradation times. Different approaches for the dataprocessing of the taylorgrams are compared, including simpleintegration of the taylorgram, curve fitting with a finite numberof Gaussian peaks, cumulant-like method and ConstrainedRegularized Linear Inversion approach. Valuable informationon the kinetics of the enzymatic/hydrolytic degradation reactions and on the degradation process can be obtained by TDA.