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(93) Production(s) de PALMERI J.
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Transport of ions in solution through single-walled carbon nanotubes
Auteur(s): Yazda K., Tahir S., Michel T., Loubet Bastien, Manghi Manoel, Bentin Jérémy, Picaud Fabien, Palmeri J., Henn F., Jourdain V.
Conference: Chemontubes 2018 (Biarritz, FR, 2018-04-22)
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A conserved mechanism drives partition complex assembly on bacterial chromosomes and plasmids
Auteur(s): Debaugny Roxanne, Sanchez Aurore, Rech Jérôme, Labourdette Delphine, Dorignac J., Geniet F., Palmeri J., Parmeggiani A., Boudsocq François, Le Berre Véronique, Walter J.-C., Bouet Jean-Yves
(Article) Publié:
Molecular Systems Biology, vol. 14 p.e8516 (2018)
Texte intégral en Openaccess :
Ref HAL: hal-01926457_v1
PMID 30446599
DOI: 10.15252/msb.20188516
WoS: 000451579500003
Exporter : BibTex | endNote
14 Citations
Résumé: Chromosome and plasmid segregation in bacteria are mostly driven by ParABS systems. These DNA partitioning machineries rely on large nucleoprotein complexes assembled on centromere sites (parS). However, the mechanism of how a few parS-bound ParB proteins nucleate the formation of highly concentrated ParB clusters remains unclear despite several proposed physico-mathematical models. We discriminated between these different models by varying some key parameters in vivo using the plasmid F partition system. We found that ‘Nucleation & caging’ is the only coherent model recapitulating in vivo data. We also showed that the stochastic self-assembly of partition complexes (i) does not directly involve ParA, (ii) results in a dynamic structure of discrete size independent of ParB concentration, and (iii) is not perturbed by active transcription but is by protein complexes. We refined the ‘Nucleation & Caging’ model and successfully applied it to the chromosomally-encoded Par system of Vibrio cholerae, indicating that this stochastic self-assembly mechanism is widely conserved from plasmids to chromosomes.
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Role of charge regulation and flow slip in the ionic conductance of nanopores: An analytical approach
Auteur(s): Manghi Manoel, Palmeri J., Yazda K., Henn F., Jourdain V.
(Article) Publié:
Physical Review E, vol. 98 p.012605 (2018)
Texte intégral en Openaccess :
Ref HAL: hal-01844602_v1
Ref Arxiv: 1712.01055
DOI: 10.1103/PhysRevE.98.012605
WoS: 000439065200005
Ref. & Cit.: NASA ADS
Exporter : BibTex | endNote
6 Citations
Résumé: The number of precise conductance measurements in nanopores is quickly growing. To clarify the dominant mechanisms at play and facilitate the characterization of such systems for which there is still no clear consensus, we propose an analytical approach to the ionic conductance in nanopores that takes into account (i) electro-osmotic effects, (ii) flow slip at the pore surface for hydrophobic nanopores, (iii) a component of the surface charge density that is modulated by the reservoir pH and salt concentration cs using a simple charge regulation model, and (iv) a fixed surface charge density that is unaffected by pH and cs . Limiting cases are explored for various ranges of salt concentration and our formula is used to fit conductance experiments found in the literature for carbon nanotubes. This approach permits us to catalog the different possible transport regimes and propose an explanation for the wide variety of currently known experimental behavior for the conductance versus cs .
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Modélisation du transport en nanofluidique (nanofiltration)
Auteur(s): Palmeri J.
Conference: Rencontre Ecotech Dessalement 2017 (Paris, FR, 2017-12-06)
Ref HAL: hal-01947949_v1
Exporter : BibTex | endNote
Résumé: Nous présentons le logiciel NanoFlux pour la modélisation des procédés industriels de nanofiltration.
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Theory and experiments on ionic transport through hydrophobic nanopores
Auteur(s): Manghi Manoel, Loubet Bastien, Palmeri J.
Conférence invité: STRONGLY COUPLED COULOMB SYSTEMS (SCCS) 2017 (Kiel, DE, 2017-07-30)
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Signature of non−electrostatic interactions in Nanofiltration
Auteur(s): Palmeri J., Deratani André
Conférence invité: Modeling Symposium, Membranes in Drinking and Industrial Water Production (MDIW2017)) (Leeuwarden, NL, 2017-02-07)
Ref HAL: hal-01947435_v1
Exporter : BibTex | endNote
Résumé: We show by modeling ionic rejection and solution flux data that a clear signature of non−electrostatic interactions emerges naturally.
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