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Micro‐CT and high‐field MRI for studying very early post‐mortem human fetal anatomy at 8 weeks of gestation 
Auteur(s): Lamouroux Audrey, Cardoso Maïda, Bottero Célia, Gallo Mathieu, Duraes Martha, Salerno Jennifer, Bertrand Martin, Rigau Valérie, Fuchs Florent, Mousty Eve, Genevieve David, Subsol Gérard, Goze-Bac C., Captier Guillaume
(Article) Publié:
Prenatal Diagnosis, vol. 44 p.3-14 (2024)
Ref HAL: lirmm-04694873_v1
DOI: 10.1002/pd.6489
Exporter : BibTex | endNote
Résumé: Objective This study involved very early post‐mortem (PM) examination of human fetal anatomy at 8 weeks of gestation (WG) using whole‐body multimodal micro‐imaging: micro‐CT and high‐field MRI (HF‐MRI). We discuss the potential place of this imaging in early first‐trimester virtual autopsy. Methods We performed micro‐CT after different contrast‐bath protocols including diffusible iodine‐based contrast‐enhanced (dice) and HF‐MRI with a 9.4 T machine with qualitative and quantitative evaluation and obtained histological sections. Results Nine fetuses were included: the crown–rump length was 10–24 mm and corresponded to 7 and 9 WG according to the Robinson formula. The Carnegie stages were 17–21. Dice micro‐CT and HF‐MRI presented high signal to noise ratio, >5, according to the Rose criterion, and for allowed anatomical phenotyping in these specimens. Imaging did not alter the histology, allowing immunostaining and pathological examination. Conclusion PM non‐destructive whole‐body multimodal micro‐imaging: dice micro‐CT and HF‐MRI allows for PM human fetal anatomy study as early as 8 WG. It paves the way to virtual autopsy in the very early first trimester. Obtaining a precision phenotype, even regarding miscarriage products, allows a reverse phenotyping to select variants of interest in genome‐wide analysis, offering potential genetic counseling for bereaved parents.
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