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(218) Production(s) de GERGELY C.
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Biofunctionalisation of Ti surfaces by means of self-assembled polypeptide films 
Auteur(s): Pelsoczi Istvan, Turzo K., Gergely C., Fazekas A., Cuisinier Frédéric
Conference: IADR meeting (Amsterdam, NL, 2005)
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Polyelectrolyte nanorings
Auteur(s): Flores H., Menchaca J-Luis, Tristan F., Gergely C., Perez Elias, Cuisinier Frédéric
Conference: International Biophysical Congress (Montpellier, FR, 2005)
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Polyelectrolyte nanoring structures : critical parameters governing formation and structural analysis
Auteur(s): Flores H., Menchaca J.L., Tristan F., Gergely C., Perez Elias, Cuisinier Frédéric
(Article) Publié:
Macromolecules, vol. 38 p.521-526 (2005)
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Structural Characterization of Self-Assembled Polypeptide Films on Titanium and Glass Surfaces by Atomic Force Microscopy
Auteur(s): Pelsoczi Istvan, Turzo Kinga, Gergely C., Fazekas Andras, Dekany Imre, Cuisinier Frédéric
(Article) Publié:
Biomacromolecules, vol. 6 p.3345 (2005)
Ref HAL: hal-00392819_v1
PMID 16283764
DOI: 10.1021/bm050360k
WoS: 000233392100061
Exporter : BibTex | endNote
9 Citations
Résumé: Chemically modified biomaterial surfaces (titanium and glass) covered with polyelectrolyte self-assembled films formed by the alternating adsorption of cationic poly-L-lysine (PLL) and anionic poly-L-glutamic acid (PGA) were structurally characterized by atomic force microscopy. Complementary information concerning the thickness and layer-by-layer growth of the films was provided by optical waveguide light-mode spectroscopy. The frequently used ex situ and the rarely used in situ build-up methods were compared. Important aspects of the industrial applicability of these films, their stability in time, and possible differences in their morphology were investigated. The films revealed a granular pattern, with grain diameters of 270 ( 87 nm for glass (up to 8 bilayers) and 303 ( 89 nm for titanium (up to 10 bilayers), independently of the build-up procedure. Both surfaces displayed a rehydration capability, the titanium surface exhibiting a better stability in time. The high roughness values observed at acidic or basic pH are related to the degree of ionization of PGA and PLL.
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Glucose oxidase adsorption on sequential adsorbed polyelectrolyte films studied by spectroscopic techniques
Auteur(s): Tristan F., Solis A., Palestino Gabriela, Gergely C., Cuisinier Frédéric, Perez Elias
Conference: 2nd Mexican Meeting on Mathematical and Experimental Physics (Mexico City, MX, 2004-09-06)
Actes de conférence: Materials Science and Applied Physics, vol. 759 p.111-120 (2005)
Ref HAL: hal-00406332_v1
Exporter : BibTex | endNote
Résumé: The adsorption of Glucose Oxidase (GOX) on layers of poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) deposited on Sequentially Adsorbed Polyelectrolyte Films (SAPFs) were studied by three different spectroscopic techniques. These techniques are: Optical Wave Light Spectroscopy (OWLS) to measure surface density; Fluorescence Resonance Energy Transfer (FRET) to verify the adsorption of GOX on the surface; and Fourier Transform Infrared Spectroscopy in Attenuated Total Reflection mode (FTIR-HATR) to inspect local structure of polyelectrolytes and GOX. Two positive and two negative polyelectrolytes are used: Cationic poly(ethyleneimine) (PEI) and poly(allylamine hydrochloride) (PAH) and anionic poly(sodium 4-styrene sulfonate) (PSS) and poly(acrylic acid) (PAA). These spectroscopic techniques do not require any labeling for GOX or SAPFs, specifically GOX and PSS are naturally fluorescent and are used as a couple donor-acceptor for the FRET technique. The SAPFs are formed by a (PEI)-(PSS/PAH)(2) film followed by (PAA/PAH)(n) bilayers. GOX is finally deposited on top of SAPFs at different values of n (n=l..5). Our results show that GOX is adsorbed on positive ended SAPFs forming a monolayer. Contrary, GOX adsorption is not observed on negative ended film polyelectrolyte. GOX stability was tested adding a positive and a negative polyelectrolyte after GOX adsorption. Protein is partially removed by PAH and PAA, with lesser force by PAA.
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Measurement of film thickness up to several hundreds of nanometers using optical waveguide lightmode spectroscopy
Auteur(s): Cuisinier Frédéric, Picart Catherine, Gergely C., Arntz Youri, Voegel Jean-Claude, Schaaf Pierre, Cuisinier Frédéric J.G., Senger Bernard
(Article) Publié:
Biosensors And Bioelectronics, vol. 20 p.553 - 561 (2004)
Texte intégral en Openaccess : 
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The Deposition of Calcium Phosphates within Polyelectrolyte Multilayer Films
Auteur(s): Cuisinier Frédéric, Gergely C., Bar-Yosef P., Govrin-Lippman R., Füredi-Milhofer Helga
(Article) Publié:
Key Engineering Materials, vol. 240-242 p.287 - 290 (2003)
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