ALEXANDRE Daniel
Fonction : chercheur CNRS
Organisme : CNRS
Chargé de Recherche
Autre(s) thème(s) de recherche ou rattachement(s) : - Physique de systèmes biologiques daniel.alexandre
umontpellier.fr
Bureau: , Etg: 2, Bât: 11 - Site : Campus Triolet
Domaines de Recherche: - Sciences du Vivant/Biologie du développement/Morphogenèse
- Sciences du Vivant/Ingénierie biomédicale/Imagerie
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Dernieres productions scientifiques :
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Fast and pure phase-shifting off-axis holographic microscopy with a digital micromirror device 
Auteur(s): Brodoline A., Alexandre D., Gross M.
(Article) Publié:
Applied Optics, vol. 61 p.4296-4302 (2022)
Texte intégral en Openaccess : 
Ref HAL: hal-03835417_v1
DOI: 10.1364/ao.452382
Exporter : BibTex | endNote
Résumé: We present a phase-shifting digital holographic microscopy technique, where a digital micromirror device enables to perform a precise phase-only shift of the reference wave. By coupling the beam into a monomode fiber, we obtain a laser mode with a constant phase shift, equally acting on all pixels of the hologram. This method has the advantage of being relatively simple and compatible with high frame rate cameras, which makes it of great interest for the observation of fast phenomena. We demonstrate the validity of the technique in an off-axis configuration by imaging living paramecia caudata.
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4D compressive sensing holographic imaging of small moving objects with multiple illuminations
Auteur(s): Brodoline A., Rawat Nitin, Alexandre D., Cubedo Nicolas, Gross M.
(Article) Publié:
Applied Optics, vol. 58 p.G127 (2019)
Texte intégral en Openaccess : 
Ref HAL: hal-02321405_v1
DOI: 10.1364/AO.58.00G127
WoS: 000502062900019
Exporter : BibTex | endNote
Résumé: In previous work [Opt. Lett. 44, 2827 (2019)], we presented a method based on digital holography and orthogonal matching pursuit, which is able to determine the 3D positions of small objects moving within a larger motionless object. Indeed, if the scattering density is sparse in direct 3D space, compressive sensing algorithms can be used. The method was validated by imaging red blood cell trajectories in the trunk vascular system of a zebrafish (Danio rerio) larva. We give here further details on the reconstruction technique and present a more robust version of the algorithm based on multiple illuminations.
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4D compressive sensing holographic microscopy imaging of small moving objects
Auteur(s): Brodoline A., Rawat Nitin, Alexandre D., Cubedo Nicolas, Gross M.
(Article) Publié:
Optics Letters, vol. 44 p.2827 (2019)
Texte intégral en Openaccess :
Ref HAL: hal-02189448_v1
DOI: 10.1364/OL.44.002827
WoS: 000469838100055
Exporter : BibTex | endNote
11 Citations
Résumé: We show that compressive sensing (CS) calculations are very ecient to reconstruct in 3D sparse objects whose 2D hologram has been recorded by digital holographic microscopy. The method is well adapted to image small scattering objects moving within a larger motionless object. This situation corresponds to red blood cells (RBCs) circulating in the vascular system of a zebrash (Danio rerio) larva. RBCs positions are imaged in 3D from a single hologram, while the RBCs trajectories, i.e. the perfused blood vessels, are imaged from a sequence of holograms. With respect to previous work (Donnarumma et al., Opt. express, 24, 26887, 2016), we get a gain of ∼ 500 in calculation speed.
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High Speed Quantitative 3D Blood Flow Imaging by Multi illumination Holographic Microscopy
Auteur(s): Brodoline A., Rawat Nitin, Donnarumma D., Alexandre D., Gross M.
(Affiches/Poster)
Journées scientifiques du LabEx NUMEV (Montpellier, FR), 2018-11-27Texte intégral en Openaccess :
Ref HAL: hal-01927083_v1
Exporter : BibTex | endNote
Résumé: A holographic microscopy method using several illuminations beams with a NA=0.5 aperture objective is proposed to image blood microcirculation in zebrafish larvae. Recent achievements in 3D imaging of blood flow are presented.
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Imagerie 4D de la circulation sanguine chez la larve du poisson-zèbre par holographie numérique en illumination multiple
Auteur(s): Brodoline A., Rawat Nithin, Alexandre D., Cubedo Nicolas, Gross M.
Conference: HOLOPHI5: 5ème rencontre francophone d’holographie numérique appliquée à la métrologie des fluides (Montpellier, FR, 2018-11-07)
Texte intégral en Openaccess :
Ref HAL: hal-01927531_v1
Exporter : BibTex | endNote
Résumé: Une configuration de microscopie holographique qui utilise trois faisceaux d'illumination est proposée. Elle permet d'imager en 3D la positions des globules rouges en mouvement à partir d'un hologramme, ainsi que la structure 3D des vaisseaux sanguins perfusé à partir d'une séquence d'hologrammes.
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